Protein Production in Insect and Mammalian Cells, Amsterdam, Netherlands

The NKI Protein Facility is highly experienced in protein production using insect and mammalian cells, and assists users with the design of expression constructs considerinbgt also solubility and domain boundaries. We collected a wide variety of expression vectors, including LIC vectors for Ligand Independent Cloning. For expression of intracellular and extracellular proteins in insect cells we have SF9 and SF21 systems. Our mammalian expression system is optimised for HEK-293T cells, but we have for instance also the glycosylation free, gnT1-deficient HEK293S variety.

 

Access to protein expression in bacteria (E. coli) is possible with the NKI Proteikn Facility, but not accessible via Instruct!

View All at Instruct